Journal: Journal of Biological Chemistry

Article Title: Effect of Human Carbonic Anhydrase II on the Activity of the Human Electrogenic Na/HCO3 Cotransporter NBCe1-A in Xenopus Oocytes

doi: 10.1074/jbc.m602181200

Figure Lengend Snippet: FIGURE 6. Immunolocalization of EGFP and CA II domains in oocytes expressing EGFP-e1-CAII or EGFP- e1. Three days after injection of cRNA encoding EGFP-e1-CAII or EGFP-e1, oocytes were prepared for immu- nocytochemistryasindicatedunder“ExperimentalProcedures.”Preparationswereincubatedovernightsimul- taneously with mouse monoclonal anti-EGFP and with rabbit polyclonal anti-human CA II. The secondary antibodies were Alexa Fluor 488-conjugated goat anti-mouse and Alexa Fluor 594-conjugated goat anti-rab- bit. A-C, Alexa Fluor 488-labeled images were taken with excitation/emission filters set at band pass 488 nm/long pass 505 nm to detect EGFP signals. D-F, Alexa Fluor 594-labeled images were taken with excitation/ emission filters set at band pass 543 nm/long pass 560 nm to detect CA II signals. These images show that EGFP-e1-CAIIandEGFP-e1arecorrectlytargetedtotheplasmamembrane.G-I,mergesoftheimagesinthefirst and second columns. G shows that the EGFP staining (green) of EGFP-e1-CAII overlapped with the CA II staining (red). J–L, Nomarski images taken simultaneously with either the EGFP or CA II images. Bar 50 m.

Article Snippet: Slides were blocked by washing (once for 15 min) in TBS 0.1% BSA 10% normal goat serum (Vector Laboratories, Burlingame, CA).We incubated the preparations with mouse monoclonal anti-EGFP (BD Biosciences; 1:100 in TBS 0.1% BSA 10% goat serum) and rabbit polyclonal anti-human CA II (Rockland, Gilbertville, PA; 1:1000 in TBS 0.1% BSA 10% goat serum) overnight.

Techniques: Expressing, Injection, Labeling, Staining

Journal: bioRxiv

Article Title: Lymph node resident memory T cells retain effector capabilities by evading lung resident memory dysfunction

doi: 10.64898/2026.02.03.703569

Figure Lengend Snippet: (A) Naive CD90.1+ OT-I T cells were transferred into naive B6 mice before intranasal infection with influenza expressing SIINFEKL (PR8-OVA). Expression of CD69 and CD103 was measured 21 days post infection on extravascular cells. (B) Quantification of CD69 + CD103 + OT-I T cells. (C) Naïve CD90.1 + OT-I Nur77-GFP T cells were transferred into mice one day before intranasal PR8-OVA infection. Nur77-GFP expression was evaluated 3-14 days later by extravascular OT-I T cells. (D)Experimental setup for (E-G). Flow cytometry plot (E) of CD90.1 + OT-I T cells isolated from indicated locations. (F-G) Longitudinal labeling of cells and quantification. (H) Experimental setup for (I-J). (I) Labeling after intravenous and intraperitoneal injection of OT-I T cells or naïve endogenous T cells. (J) Expression of CD69 and CD103. Naïve= CD8 + CD90.1 - CD44 low . Each point represents a mouse and N=2-5 mice per experiment. Data are representative (A) or are pooled from at least two experiments (n=2–4/group), except for day 113 in (B), which reflect a single experiment.

Article Snippet: Measurements were obtained in Flow Cytometry (FACS) Staining Buffer (Rockland, MB-086-0500) at room temperature for about 30 minutes per sample and channels were flushed with buffer in between samples.

Techniques: Infection, Expressing, Flow Cytometry, Isolation, Labeling, Injection

Journal: bioRxiv

Article Title: Lymph node resident memory T cells retain effector capabilities by evading lung resident memory dysfunction

doi: 10.64898/2026.02.03.703569

Figure Lengend Snippet: (A)Chromatin accessibility with gene expression (right) and linked chromatin peaks (bottom) at the Gzmb locus. (B) Protein expression of GranzymeB as measured by flow cytometry. (C-D) Same as (A-B) but for the Pdcd1 locus. Protein expression on OT-I T cells is 49 days post infection where each dot represents one mouse. (J and L) Data are from one experiment and representative of at least two experiments with N=3. (E-F) Surface expression of PD-1 and TIM3 at 7 days post infection. (G) Transcription factor motif footprint analysis from dataset. (H) Inferred eRegulons identified by SCENIC+ across memory T cells after influenza infection. (I-K) Legend (left) for network plots illustrating inferred transcription factor relationships to target genes. Transcription factor and gene relationship is denoted by color of line represents activation (red) or repression (blue).

Article Snippet: Measurements were obtained in Flow Cytometry (FACS) Staining Buffer (Rockland, MB-086-0500) at room temperature for about 30 minutes per sample and channels were flushed with buffer in between samples.

Techniques: Gene Expression, Expressing, Flow Cytometry, Infection, Activation Assay